Composite

Part:BBa_K1030008:Design

Designed by: Wei Huang   Group: iGEM13_SUSTC-Shenzhen-A   (2013-09-16)


Zeo-resistant gene + GFPuv


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We want to use signalling molecule produced by E coli to tune zeo-r expression, and its growth rate withzeo presents.


Source

Zeo is from a commercial plasmid. GFPuv is from iGEM

References